Interleukin 1 alpha (IL1A), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse IL1A (NP_034684.2) (Ser 115-Ser 270), with an initial Met at the N-terminus.

Activity:
1. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is typically 2-10 pg/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized mouse IL1A at 10 µg/ml (100 µl/well) can bind mouse IL1R1, The EC₅₀ of mouse IL1R1 is 0.13 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.