Dickkopf 1 homolog (Xenopus laevis) (DKK1), recombinant rhesus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its ability to inhibit WNT3A-induced alkaline phosphatase production by C3H10T1/2 cells. The ED₅₀ for this effect is approximately 0.1-0.4 µg/ml in the presence of 100 ng/ml of mouse Wnt3a.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.