This is a purified rabbit anti-human ERK monoclonal antibody (mAb) that can be used in validated applications such as western blotting (WB), indirect ELISA, immunocytochemistry (ICC) and flow cytometry (FC).
ABfinity™ antibody characteristics include:
• APPLICATIONS: This ERK Monoclonal Antibody, ABfinity™ Recombinant has been validated for use in western blotting, indirect ELISA, immunocytochemistry, and flow cyotmetry.
• HOST SPECIES AND ISOTYPE: This is a recombinant rabbit monoclonal IgG against human ERK.
• CLONE ID OF MONOCLONAL (mAb): The monoclonal antibody clone is 3H4L13.
• REACTIVITY: Along with human ERK, this monoclonal antibody is predicted to react with mouse, rat, monkey, equine, and bovine.
• PRODUCT SIZE: 100 µg
Specific for ERK
The erks are abundantly expressed in the neurons in a mature central nervous system. ERK1 and ERK 2 are activated by growth factors, hormones and phorbol esters. Phosphorylation of ERK occurs through a series of events which include phosphorylations by kinases which activate other kinases and finally bring about the phosphorylation of erk. Activated ERK plays a role in control of cell proliferation, cell differentiation, and regulation of the cytoskeleton. Once the ERK is activated, it phosphorylates cytoplasmic signaling molecules, cell surface receptors, transcription factors, and microtubule associated proteins.
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![Indirect ELISA of...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/701183_Indirect%20ELISA_marketing.JPG","Indirect ELISA of ERK1 ABfinity™ Recombinant Rabbit monoclonal Antibody – Purified","Indirect ELISA was performed using various dilutions of ERK1 ABfinity™ Recombinant Rabbit monoclonal Antibody (Cat. No.701183) to detect ERK1 recombinant protein coated onto the plate. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody was determined.");)
![Western Blot anal...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/701183%20ERK1%20web.jpg","Western Blot analysis of ERK1 ABfinity™ Recombinant Rabbit monoclonal Antibody – Purified","Western blot analysis was performed on whole cell extracts from U87MG, MCF-7, Hela, HepG2, MDAMB-231, PC12, MDAMB-453 and Jurkat - (30µg each). Endogenous level of ERK1 was detected at ~42 kDa using ERK1 ABfinity™ Recombinant Rabbit monoclonal Antibody at 1 µg/mL. The blot was developed using chemiluminescence (ECL) method.");)
![Flow cytometry an...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/701183%20ERK1%20WEB%20Flow.jpg","Flow cytometry analysis of Hela cells labelled with ABfinity™ ERK 1 Recombinant Rabbit monoclonal Antibody","Fixed and permeabilized Hela cells were labelled with ABfinity™ ERK 1 Recombinant Rabbit monoclonal Antibody, followed by Alexa Fluor 488 goat anti Rabbit IgG staining (right peak). To confirm specificity, the cells were labeled with an isotype control and stained using Alexa Fluor® 488 goat anti-rabbit IgG (left peak).");)
![Immunocytochemist...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/701183%20ERK%20final.jpg","Immunocytochemistry analysis of ERK1 ABfinity™ Recombinant Rabbit monoclonal Antibody – Purified","Immunocytochemistry analysis of Hela cells stained with ERK1 ABfinity™ Recombinant Rabbit monoclonal Antibody a: Alexa Fluor® 488 goat anti-rabbit was used as secondary (green). b: DAPI was used to stain the nucleus (blue) and c: Alexa Fluor® 594 phalloidin was used to stain actin (red). d: Composite image of cells showing cytoplasmic localization of ERK1.");)
