This is a purified rabbit anti-human TIMP4 monoclonal antibody (mAb) that can be used in validated applications such as western blotting (WB), indirect ELISA, immunocytochemistry (ICC), and flow cytometry (FC).
ABfinity™ antibody characteristics include:
• APPLICATIONS: This TIMP4 Monoclonal Antibody, ABfinity™ Recombinant has been validated for use in western blotting, indirect ELISA, immunocytochemistry, and flow cyotmetry.
• HOST SPECIES AND ISOTYPE: This is a recombinant rabbit monoclonal IgG against human TIMP4.
• CLONE ID OF MONOCLONAL (mAb): The monoclonal antibody clone is 13H4L13.
• REACTIVITY: Along with human TIMP4, this monoclonal antibody is predicted to react with monkey, rabbit, bovine, canine, bat, equine, rat, and mouse.
• PRODUCT SIZE: 100 µg
Specific for TIMP4
Tissue inhibitor of metalloproteinase 4 (TIMP4) is encoded by the gene TIMP4 located on chromosome 3. It belongs to the family of TIMP genes and is known to inhibit the activity of matrix metalloproteinases. Matrix metalloproteinases (MMP) are a group of proteinases that degrade the components of extracellular matrix. TIMP4 irreversibly binds to MMPs and inactivates them with the help of zinc as a cofactor. The netrin domain of TIMP4 is involved in platelet recruitment and aggregation. Other biological functions include response to cytokine and peptide hormone stimuli and involvement with the ovulation cycle and CNS development.
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![Western Blot anal...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/701317%20TIMP4%20WEB.jpg","Western Blot analysis of TIMP4 ABfinity™ Recombinant Rabbit monoclonal Antibody – Purified","Western blot analysis was performed on whole cell extracts from MBAMB231 (30 ?g). Endogenous level of TIMP4 was detected at ~25 kDa using TIMP4 ABfinity™ Recombinant Rabbit monoclonal Antibody at 2 ?g/mL. The blot was developed using chemiluminescence (ECL) method.");)
![Indirect ELISA of...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/701317_Indirect%20ELISA_marketing.JPG","Indirect ELISA of TIMP4 ABfinity™ Recombinant Rabbit monoclonal Antibody – Purified","Indirect ELISA was performed using various dilutions of TIMP4 ABfinity™ Recombinant Rabbit monoclonal Antibody (Cat. No.701317) to detect endogenous TIMP4 in Hela lysate, coated onto the plate. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody was determined.");)
![Immunocytochemist...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/701317%20timp4_mono_final.jpg","Immunocytochemistry analysis of TIMP4 ABfinity™ Recombinant Rabbit monoclonal Antibody – Purified","Immunocytochemistry analysis of U20S cells stained with TIMP4 ABfinity™ Recombinant Rabbit monoclonal Antibody a: Alexa Fluor® 488 goat anti-rabbit was used as secondary (green). b: DAPI was used to stain the nucleus (blue) and c: Alexa Fluor® 594 phalloidin was used to stain actin (red). d: Composite image of cells showing cytoplasmic localization of TIMP4.");)
![Flow cytometry an...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/701317%20TIMP4%20web%20Flow.jpg","Flow cytometry analysis of Hela cells labelled with ABfinity™ TIMP4 Recombinant Rabbit monoclonal Antibody","Fixed and permeabilized Hela cells were labelled with ABfinity™ TIMP4 Recombinant Rabbit monoclonal Antibody, followed by Alexa Fluor 488 goat anti Rabbit IgG staining (right peak). To confirm specificity, the cells were labeled with an isotype control and stained using Alexa Fluor® 488 goat anti-rabbit IgG (left peak).");)
