Kinase insert domain receptor (KDR/CD309), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 764) of human KDR (NP_002244.1) fused to the Fc region of human IgG1 at the C-terminus.

Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human VEGFA at 1.25 µg/ml (100 µl/well) can bind KDR with a linear range of 1.25-40.0 ng/ml.
2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED₅₀ for this effect is typically 9.6 ng/mL in the presence of 5-20 ng/mL recombinant human VEGF165.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.