Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

• Supports growth of suspension 293 cultures to densities of over 1.6 x 10⁷ cells/mL, with >95% cell viability
• Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine™ 293 transfection reagent
• Enables sustained, high level expression of high-density transiently-transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats from less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
• Does not contain phenol red

Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human- or animal-origin components. The medium is not recommended for adherent cell cultures.