NuPAGE® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. NuPAGE® Antioxidant is added to the running buffer in the upper (cathode) buffer chamber when performing protein gel electrophoresis under reducing conditions. NuPAGE® Antioxidant is provided in a 15 mL size.

NuPAGE® Antioxidant must be used with samples reduced using NuPAGE® Sample Reducing Agent or other reducing agents. NuPAGE® Antioxidant is optimized for the neutral pH of NuPAGE® Novex® gels and is not compatible with other gel chemistries. NuPAGE® Antioxidant may also be added to NuPAGE® Transfer Buffer to enhance transfer of proteins to membranes.

NuPAGE® Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent reoxidation of sensitive amino acids such as methionine and tryptophan.