InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll:
- Stain proteins fused to an oligohistidine sequence
- Eliminate western blotting-detect His-tagged proteins directly in the gel
- Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner
- Visualize results in under 3 hours
Convenienty provided as one ready-to-use solution, InVision™ His-tag In-gel Stain does not require mixing, dilution, or solution preparation.Fast and Easy StainingThe InVision™ His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under 3 hours, nanogram levels of His-tagged fusion protein can be detected (Figure 1). After documenting your InVision™ Stain signal, total protein staining can be performed.