Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic abnormalities and maintains the ability of iPSCs to differentiate into all three germ line lineages.
• Consistent - Reduced variability compared to existing feeder-free culture media
• Robust - Reliable and robust cultures with a xeno-free, cGMP, 8-component medium
• Cost effective - Economical and scalable PSC culture compared to other feeder-free media

Note: The "prototype" status of this product means that the product is currently undergoing real time stability studies in compliance with cGMP regulations.

Reduced Variability
Essential 8™ Medium is xeno-free and contains only the eight essential components needed for stem cell culture. Unlike other media that contain over 20 highly variable ingredients, Essential 8™ Medium is produced under cGMP and has an optimized formulation and growth factor levels to help ensure maximum cell health, pluripotency, and growth, with minimal variability.

Reliable and Robust Cultures
Essential 8™ Medium has been show to support pluripotent stem cell growth and provide cultures with superior morphology and growth kinetics compared to other feeder systems.

Cost Effective
Essential 8™ Medium is provided in a convenient two component kit (500 ml basal & 10 ml supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs).

Essential 8™ Medium is commercialized in partnership with Cellular Dynamics International.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Reference:
Chemically defined conditions for human iPSC derivation and culture; Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Nat Methods. 2011 Apr 10.