Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.
Save Time and Money with Your Sample Screening
This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR systems enable researchers to inexpensively and efficiently:
• Screen samples for relative protein thermal stability
• Screen molecules (e.g. small molecule drug candidates, fragment-based libraries, and antibodies) for ligand binding to proteins in a high-throughput manner
• Screen samples for stability changes after protein point mutations or modifications
• Systematically identify suitable⁄optimal buffer conditions for the measurement of protein-ligand interactions, production of stable protein preparations, protein crystallization, and storage of proteins
• Quickly QC protein preps to ensure protein integrity and⁄or purity
Two Kits to Choose From
The Protein Thermal Shift™ Dye Kit contains the dye and buffer for performing protein thermal shift experiments, enough for up to 2000 reactions. The Protein Thermal Shift™ Starter Kit contains the Dye Kit plus a control protein and control ligand for test experiments and troubleshooting. The Protein Thermal Shift™ Starter Kit components are packaged in two boxes, one to be stored at -20°C and the other at 4°C, for maximum flexibility in reagent formulation.
Simplify Analysis with Protein Thermal Shift™ Software
Our complete Protein Thermal Shift™ solution includes analysis software which is compatible with run files generated from five of our real-time PCR systems: StepOne™ Real-Time PCR System, StepOnePlus™ Real-Time PCR system, 7500 Fast Real-Time PCR System, and ViiA™7 Real-Time PCR System.
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![An Example of Pro...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/4462263_635_2.jpg","An Example of Protein Thermal Shift™ Data","Example data for a buffer screen experiment processed with the Protein Thermal Shift® software. Three different buffers ranging in pH from 6.0-7.5 were screened using the StepOnePlus® Real-Time PCR System to define the optimal pH range for increased thermal stability of the protein being tested. The green trace is the sample run at pH=7.5, red is at pH 6.0 and blue is pH 7.0 (highest T<sub>m</sub>=most stable). Samples were run in quadruplicate. The upper trace is the fluorescence measurement, and the lower trace is the derivative trace. Both traces are used to determine the melting temperature (T<sub>m</sub>).");)
