Clone/PAD
ZMD.667
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoserines 33 and 37 of human β-catenin.
Specificity
Specific for the ~83 kDa β-catenin phosphorylated at serines 33 and 37.
Reactivity
The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with mouse, rat and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used
Applications
Western blotting. Other applications have not been tested
References
1. Ding Y, Dale, T, Trends Biochem Sci 27:327-329, 2002.
2. Polakis P, Genes Dev 14:1837-1851, 2000.
3. Liu C, et al., Cell 108:837-847, 2002.
4. Wang ZH, et al., Cancer Res 63:5234-5235, 2003.
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![Western blot anal...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/48-6000-beta-catenin-psps-3.jpg","Western blot analysis of human embryonic kidney cell (HEK) lysate using rabbit anti-beta catenin [pSpS33/37] antibody (Catalog no. 48-6000). Data demonstrate specific immunolabeling of the ~83 kDa ß-catenin phosphorylated at serine 33 and serine 37 (Upper Panel) and a pan ß-catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3ß knocked down by SiRNA.","Western blot analysis of human embryonic kidney cell (HEK) lysate using rabbit anti-beta catenin [pSpS33/37] antibody (Catalog no. 48-6000). Data demonstrate specific immunolabeling of the ~83 kDa ß-catenin phosphorylated at serine 33 and serine 37 (Upper Panel) and a pan ß-catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3ß knocked down by SiRNA.");)
