This rabbit anti-Cdc4 polyclonal antibody is specific to human Cdc4 protein and expected to react with mouse (not tested). Cdc4 polyclonal antibody recognizes the expressed product of the Fbxw7 gene, also known as FBXW7. Cdc4 is an inhibitor of Notch signaling that targets Notch for ubiquitin-mediated degradation after a nuclear phosphorylation event. Validated applications for Cdc4 polyclonal antibody are Western blotting and immunoprecipitation.

Applications: validated for Western blot and immunoprecipitation
Host species and isotype: rabbit IgG
Polyclonal Antibody Designation: ZMD.432
Reactivity: human (mouse) Cdc4
Product size: 100 µg pack size

The F-box protein family is characterized by an approximately 40 amino acid motif the F-box.F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. They constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Cdc4 is one member of this family, which is also known as AGO, FBW7, FBX30 or SEL10. CDC4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Cdc4 proteins of amino acid lengths varying from 553 aa to 707 aa in length have been reported. Cdc4 is an inhibitor of Notch signaling that targets Notch for ubiquitin-mediated degradation after a nuclear phosphorylation event. Cdc4 interacts with presenilin 1, facilitates its ubiquitination, and alters A-beta peptide production. Thus, it may be important for Alzheimer's disease. Mutations of the CDC4 gene are detected in ovarian and breast cancer cell lines, and the CDC4 gene may also be involved in the pathogenesis of human pancreatic and endometrial cancers.