NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent.

For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X).

Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample Buffer is a highly viscous and concentrated solution. NuPAGE® LDS buffer contains twice the amount of LDS vs. SDS in Novex® Tris-Glycine SDS Sample Buffer or Tricine SDS Sample Buffer. NuPAGE® LDS Sample Buffer also contains a higher concentration glycerol.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.