Illumina® TotalPrep™ RNA Amplification Kit

(Ambion®)

Description

The Ambion® Illumina® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The new TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous Illumina® RNA Amplification Kit.

Experience these Improvements:

• Improved first-strand cDNA synthesis using ArrayScript™ reverse transcriptase
• Biotinylated UTP allows ease of use and optimal labeling
• Maximization of cRNA yield using MEGAscript® IVT technology
• Significantly improved sensitivity starting with just 50 ng of input RNA

New Improvements for a Tested and Reliable Amplification Procedure
The TotalPrep™ RNA Amplification Kit is based on the RNA amplification protocol developed in the laboratory of James Eberwine (see reference). The procedure consists of reverse transcription with an oligo(dT) primer bearing a T7 promoter (TTTT-P_T7) using ArrayScript™ (Ambion®), a proprietary reverse transcriptase (RT) engineered to produce higher yields of first strand cDNA than wild type enzymes. ArrayScript™ catalyzes the synthesis of virtually full-length cDNA, which is the best way to ensure production of reproducible microarray samples. The cDNA then undergoes second-strand synthesis and clean-up to become a template for in vitro transcription with T7 RNA Polymerase. To maximize cRNA yield, Ambion's proprietary MEGAscript® in vitro transcription (IVT) technology, along with biotin UTP (provided in the kit), is used to generate hundreds to thousands of biotinylated, antisense RNA copies of each mRNA in a sample.

Catalog Number

AMIL1791

Size: 24 reactions

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List Price: (INR) 68,839.00; My Price & Availability
Your Price: (INR) 68,839.00

Qty

This product is available at a local Supply Center.

Specifications

General Specifications

System:	Illumina Sentrix®
Product Size:	24 reactions
Labeling Method:	Direct Labeling
cGMP Compliance:	non-cGMP Compliant
Includes Label or Dye:	Yes
Reverse Transcriptase:	ArrayScript™ Reverse Transcriptase
Sample Type (Specific):	RNA (Poly(A)+ Transcripts), Total RNA
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Contents and Storage

Store at -20 °C: T7 Oligo(dT) Primer, 10X First Strand Buffer, dNTP Mix, RNase Inhibitor, ArrayScript™, 10X Second Strand Buffer, DNA Polymerase, RNase H, T7 10X Reaction Buffer, T7 Enzyme Mix, Biotin-NTP Mix, Nuclease-free Water, 10 µL Control RNA (1 mg⁄mL HeLa total RNA).

Store at Room Temp: Nuclease-free Water, Wash Buffer.

Store at 4°C or Room Temp: cDNA Binding Buffer, cRNA Binding Buffer, cRNA Filter Cartridges, cRNA Collection Tubes, cDNA Filter Cartridges and Tubes, cDNA Elution Tubes.