One Shot® TOP10 E. coli are convenient to use chemically competent cells quality controlled for a transformation efficiency of 1 x 10⁹ cfu/ µg plasmid DNA. One Shot® TOP10 Chemically Competent cells are the same competent cells that come with many of our cloning kits and are ideal for high-efficiency cloning and plasmid propagation.
• Maximizes cloning efficiency in single tube format
• Enhanced genomic DNA cloning capabilities

Easy-to-use, Single Tube Format
One Shot® TOP10 E. coli are provided at a transformation efficiency of 1 x 10⁹ cfu/ µg supercoiled DNA and allow stable replication of high-copy number plasmids, making them ideal for high-efficiency cloning and plasmid propagation. The single tube, single use, format allows for all steps of transformation up to plating to take place in the same tube, thereby helping to save time and prevent contamination.

Versatile Cloning Capabilities
One Shot® TOP10 E. coli cells are similar to the DH10B™ strain, and offer the following features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Genotype:
F- mcrA Δ( mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ( araleu)7697 galU galK rpsL (StrR) endA1 nupG

Find the Strain and Format That You Need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require high-throughput transformation choose from our collection of MultiShot™ formatted comp cells.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.