| Color: |
Orange |
| Platform: |
Fluorescence Microscope |
| Product Size: |
1 mg |
| Detection Method: |
Fluorescent |
| Cell Permeability: |
Cell-Impermeant |
| Sub-Cellular Localization: |
Nucleic Acids, Nucleus |
| Excitation⁄Emission (nm): |
528⁄617 |
| Shipping Condition: |
Room Temperature |
| Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures. |
Store in freezer (-5 to -30°C) and protect from light.
Fluorescence Ex/Em spectra of ethidium homodimer-1 bound to DNA.
Note: Use lot number only (e.g., "K18126").
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![Rat lung cells. L...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001402.jpg","Rat lung cells. LIVE/DEAD® Viability/Cytotoxicity Kit, ethidium homodimer-1 and calcein AM.","The components of the LIVE/DEAD® Viability/Cytotoxicity Kit (Cat. no. L3224) were perfused into the vasculature of a rat lung. Cells with compromised membranes exhibit red-fluorescence from the live-cell–impermeant nucleic acid stain ethidium homodimer-1 (Cat. no. E1169). Cells with intact cell membranes are able to use nonspecific cytosolic esterases to convert nonfluorescent calcein AM (Cat. no. C1430, C3099, C3100MP) into bright green-fluorescent calcein (Cat. no. C481). Images and reconstructions were performed on a Bio-Rad laser scanning confocal microscope. Image contributed by Lance Rodenkirch, W. M. Keck Laboratory for Biological Imaging, University of Wisconsin.");)
![Rat mesenteric ar...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000684.jpg","Rat mesenteric artery. Ethidium homodimer–1.","Rat mesenteric artery stained with ethidium homodimer–1 (E1169). The artery was bathed in Krebs\\\)
![Mouse 3T3 cells. ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000685.jpg","Mouse 3T3 cells. Ethidium homodimer–1 and BODIPY® FL phallacidin.","Mouse 3T3 cells stained with ethidium homodimer–1 (Cat. no. E1169) and BODIPY® FL phallacidin (Cat. no. B607). The nucleic acids in these cells were stained red fluorescent by ethidium homodimer–1, whereas the actin filaments, probed with BODIPY® FL phallacidin, are green fluorescent. The image was photographed in a single exposure through an Omega Optical longpass filter appropriate for fluorescein.");)
