ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR. This dye has been qualified for use on the ABI PRISM® 7700 with Platinum® Quantitative PCR SuperMix-UDG® , Platinum® Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript® III Platinum® One-Step qRT-PCR Kit. ROX Reference Dye:
- Normalizes non-PCR-related fluctuations in fluorescence
- Provides a stable baseline for multiplex quantitative PCR and RT-PCR
- Uses existing ROX spectral settings, eliminating the need to re-calibrate the thermal cycler
