MuLV Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. It can be used for the preparation of cDNA libraries, for first-strand synthesis in RT-PCR, or in dideoxy sequencing reactions in place of Klenow enzyme, for G+C–rich regions. MuLV Reverse Transcriptase lacks endonuclease activity and has extremely low RNase H activity compared to AMV reverse transcriptase.