(Invitrogen™)
| Promoter: |
CMV |
| Protein Tag or Fusion: |
V5 Epitope Tag |
| Constitutive or Inducible System: |
Constitutive |
| Delivery Method: |
Lentiviral |
| Cloning Method: |
Directional TOPO® |
| Key Function: |
Viral Expression |
| Selection Agent (Eukaryotic): |
Blasticidin |
| Vector Type: |
Directional TOPO Vectors |
| Product Size: |
1 kit |
| Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures. |
pLenti6⁄V5™ Directional TOPO® Cloning Kit:
• pLenti6⁄V5™ Directional TOPO® Reagents: -20°C
• One Shot® Stbl3™ Chemically Competent E. coli: -80°C
ViraPower™ Bsd Lentiviral Support Kit:
• ViraPower™ Packaging Mix: -20°C
• Lipofectamine® 2000: 4°C (DO NOT freeze)
• Blasticidin: -20°C
• 293FT Cell Line: liquid nitrogen
Note: Use lot number only (e.g., "K18126").
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| Cell Line Name | Organism Type | Tissue |
|---|---|---|
| Mono-Mac-6 cells | Human | Blood |
| LNCaP | Human | Prostate |
| 293 | Human | Kidney |
| Beas-2B | Human | Lung |
| PC-3 | Human | Prostate |
| Fibroblasts (normal) | Human | Fibroblast |
| Neurons (embryonic cortical) | Rat | Brain |
| HCA-7 | Human | Colon |
| 3Y1 | Rat | Fibroblast |
| Bone marrow cells | Mouse | Bone marrow |
| Epithelial cells (SRA01/04) | Human | Epithelial |
| Splenocytes (B cells T2) | Mouse | Spleen |
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