NuPAGE® LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with NuPAGE® Novex® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X).

Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample Buffer is a highly viscous and concentrated solution—it contains twice the amount of LDS compared to the amount of SDS in Novex® Tris-Glycine SDS Sample Buffer or in Tricine SDS Sample Buffer. NuPAGE® LDS Sample Buffer also contains a higher concentration glycerol.