- Selectively stains F-actin
- Outstanding fluorescence performance
- Excitation/Emission: 591/608 nm
- Superior to antibody staining
- Optimal for fixed and permeabilized samples
Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Texas Red®-X provides red fluorescence with superior brightness. Demonstrating very little nonspecific staining, Texas Red®-X phalloidin allows high-contrast discrimination of actin staining.
Use in Multiple Applications
Texas Red®-X phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Texas Red®-X phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Links
- Read about labeling F-Actin with phallotoxins.
- Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.
![Ovarioles from Dr...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001907.jpg","Ovarioles from Drosophila melanogaster. ","Filamentous actin (F-actin), localized near the membranes and the intercellular cytoplasmic bridges or ring canals of D. melanogaster ovarioles, was stained with red-fluorescent Texas Red®-X phalloidin (Cat. No. T7471). Nuclei of the follicle cells surrounding the ovarioles and nuclei of the nurse cells within the egg chambers were stained with Hoechst 33258 (Cat. No. H1398, H3569, H21491). The signal from the Hoechst 33258 dye has been pseudocolored green. The image was contributed by Ming-Der Lin, National Taiwan University.");)
![Cytoskeleton of a...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001217.jpg","Cytoskeleton of a mixed population of granule neurons and glial cells. Texas Red®-X phalloidin and Alexa Fluor® 488 goat anti–mouse IgG.","Confocal micrograph of the cytoskeleton of a mixed population of granule neurons and glial cells. The F-actin was stained with red-fluorescent Texas Red®-X phalloidin (Cat. no. T7471). The microtubules were detected with a mouse monoclonal anti–ß-tubulin primary antibody and subsequently visualized with the green-fluorescent Alexa Fluor® 488 goat anti–mouse IgG antibody (Cat. no. A11001). The image was contributed by Jonathan Zmuda, Immunomatrix, Inc.");)
![UV-irradiated mun...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001984.jpg","UV-irradiated muntjac skin fibroblasts showing mitochondrial damage.","UV-irradiated muntjac skin fibroblasts showing mitochondrial damage. Fixed, permeabilized cells were stained with Texas Red®-X phalloidin (Cat. no. T7471) to visualize F-actin in the cytoskeleton. Endogenous biotin in the mitochondria was detected with Alexa Fluor® 488 streptavidin (Cat. no. S11223). Nuclei were counterstained with DAPI (Cat. no. D1306, D3571, D21490).");)
![Bovine pulmonary ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001827.jpg","Bovine pulmonary artery endothelial cells (BPAEC). FluoCells prepared slide #2.","FluoCells prepared slide #2 (Cat. no. F14781), which shows bovine pulmonary artery endothelial cells (BPAEC) that have been stained with an anti–ß-tubulin mouse monoclonal antibody in conjunction with BODIPY FL goat anti–mouse IgG (Cat. no. B2752) for labeling microtubules, Texas Red-X phalloidin (Cat. no. T7471) for labeling F-actin and DAPI (Cat. no. D1306, D3571, D21490) for labeling nuclei. This multiple-exposure image was acquired using bandpass optical filter sets appropriate for DAPI, fluorescein and Texas Red dye.");)
![Bovine pulmonary ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000997.jpg","Bovine pulmonary artery endothelial cells. FluoCells® prepared slide #2.","FluoCells® prepared slide #2 (Cat. no. F14781) showing bovine pulmonary artery endothelial cells probed with anti–bovine a-tubulin mouse monoclonal 236-10501 (Cat. no. A11126) and visualized with BODIPY® FL goat anti–mouse IgG (Cat. no. B2752). The actin filaments were then labeled with Texas Red®-X phalloidin (Cat. no. T7471) and the nuclei were counterstained with DAPI (Cat. no. D1306, D3571). The multiple-exposure image was acquired using bandpass filters appropriate for Texas Red® dye, fluorescein and DAPI.");)
![The relative phot...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001051.jpg","The relative photobleaching rates of fluorescein and Texas Red® fluorophores with buffer alone or after treatment with the ProLong® antifade reagent.","The relative photobleaching rates of fluorescein and Texas Red® fluorophores with buffer alone (right series) or after treatment with the ProLong® antifade reagent (left series). Bovine pulmonary artery endothelial cells were fixed, permeabilized and labeled with Texas Red®-X phalloidin (Cat. no. T7471), which labels F-actin, and with a mouse monoclonal anti–ß-tubulin antibody and fluorescein goat anti–mouse IgG antibody (Cat. no. F2761), which label microtubules. Images were acquired at appropriate wavelengths using a cooled CCD camera.");)
![Fixed, permeabili...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001834.jpg","Fixed, permeabilized bovine pulmonary artery endothelial cells labeled with Texas Red®-X phalloidin.","Fixed, permeabilized bovine pulmonary artery endothelial cells were labeled with Texas Red®-X phalloidin (Cat. no. T7471), which stains F-actin, and counterstained with DAPI (Cat. no. D1306, D3571, D21490). The panels show the unprocessed image (upper panel), after deconvolution (middle panel) and after deconvolution and three-dimensional reconstruction (lower panel). The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/). 3-D reconstruction was performed using Imaris software (Bitplane AG, http://www.bitplane.com/).");)
![Mouse Anti-Alpha ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/Mouse_Anti_Alpha_Tubulin_Monoclonal_Antibody05.jpg","Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)","Multicolor cytoskeletal staining of a bovine pulmonary artery endothelial cell. Actin filaments were detected using Texas Red®-X phalloidin (Cat. no. T7471). Tubulin was labeled with a primary antibody directed against a-tubulin (Cat. no. A11126) and visualized using green-fluorescent BODIPY® FL goat anti–mouse IgG (Cat. no. B2752). Nuclei were counterstained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490).");)
