(Molecular Probes®)
![Adult zebrafish i...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000698.jpg","Adult zebrafish intestine. ELF 97 Endogenous Phosphatase Detection Kit.","Lightly fixed adult zebrafish intestine stained with the substrate contained in the ELF 97 Endogenous Phosphatase Detection Kit (E6601). This kit\\\)
Adult zebrafish intestine. ELF 97 Endogenous Phosphatase Detection Kit.
![Exponentially gro...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000511.jpg","Exponentially growing promyelocytic leukemia (HL60) cells. Hoechst 33258 and ChromaTide BODIPY FL-14-dUTP.","Detection of BrdU-incorporating cells using the SBIP (Strand Breaks Induced by Photolysis) technique. Exponentially growing human promyelocytic leukemia (HL60) cells were incubated with 20 µM BrdU (B23151) for 40 minutes and then with 20 µg/mL Hoechst 33258 (H1398, H3569, H21491) in the presence of 2% DMSO for an additional 20 minutes. After this incubation, the cells were exposed to 300 nm UV light for 5 minutes to selectively photolyze DNA that contained the incorporated BrdU, and then fixed in 70% ethanol. Subsequent incubation of the permeabilized cells with the ChromaTide BODIPY FL-14-dUTP (C7614) in the presence of exogenous terminal deoxynucleotidyl transferase resulted in incorporation of the fluorophore into DNA strand breaks, thereby labeling the S-phase cells. The DNA was counterstained with the red-fluorescent nucleic acid stain propidium iodide (P1304MP, P3566, P21493); therefore, the BODIPY FL dyeâlabeled DNA appears yellow. The image was contributed by Zbigniew Darzynkiewicz, Cancer Research Institute, New York Medical College.");)
Exponentially growing promyelocytic leukemia (HL60) cells. Hoechst 33258 and ...
![Jurkat human T ce...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000887.jpg","Jurkat human T cell leukemia cells. Alexa Fluor 488 annexin V and propidium iodide.","Jurkat human T cell leukemia cells treated with 1 µM camptothecin. The externalized phosphatidylserine, a characteristic of early-stage apoptotic cells, was detected with Alexa Fluor 488 annexin V (A13201). The late-stage apoptotic and necrotic cells were stained with propidium iodide (P1304MP, P3566, P21493). The image was acquired using bandpass filters appropriate for fluorescein.");)
Jurkat human T cell leukemia cells. Alexa Fluor 488 annexin V and propidium i...
![Human metaphase c...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000486.jpg","Human metaphase chromosomes. Propidium iodide.","Human metaphase chromosomes stained with propidium iodide (Cat. no. P1304MP, P3566, P21493).");)
![A human pancreati...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g001710.jpg","A human pancreatic islet stained with Newport Green⢠DCF diacetate and propidium iodide.","A human pancreatic islet stained with Newport Green⢠DCF diacetate (Cat. no. N7991) and propidium iodide (Cat. no. P1304MP, P3566, P21493). The image represents a projection of 60 optical sections acquired at 2 µm intervals by confocal laser-scanning microscopy. The green-fluorescent Newport Green⢠DCF indicator identifies viable insulin-producing Ã-cells via binding to intracellular zinc. Dead cells are identified by red-fluorescent propidium iodide staining. Image contributed by Brigitte Vandewalle, University of Lille, and reproduced with permission from J Histochem Cytochem 49, 519 (2001).");)
A human pancreatic islet stained with Newport Green⢠DCF diacetate and propid...
![Jurkat cells. YO...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000964.jpg","Jurkat cells. YO-PRO®-1 dye and propidium iodide, components of the Membrane Permeability/Dead Cell Apoptosis Kit.","Apoptosis induced in Jurkat cells with 10 µM camptothecin. The cells were then treated with the reagents in the Membrane Permeability/Dead Cell Apoptosis Kit (Cat. no. V13243). Apoptotic cell nuclei were labeled with YO-PRO®-1 dye (green) (Cat. no. Y3603). Necrotic cells were detected with propidium iodide (red) (Cat. no. P1304MP, P3566, P21493).");)
Jurkat cells. YO-PRO®-1 dye and propidium iodide, components of the Membrane...
![Human keratinocyt...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000694.jpg","Human keratinocytes. Fluorescein concanavalin A and propidium iodide.","Apoptotic human keratinocytes that were fixed with paraformaldehyde and stained with fluorescein concanavalin A (Cat. no. C827). The cells were subsequently permeabilized with acetone, stained with propidium iodide (Cat. no. P1304MP, P3566, P21493) and visualized by confocal laser-scanning microscopy. This photomicrograph clearly shows that the green-fluorescent lectin staining outlines the apoptotic surface blebs, whereas the red-fluorescent propidium iodide stains the interior of the blebs. Image contributed by Livia Casciola-Rosen and Antony Rose, Johns Hopkins University. Reproduced from J Exp Med 179, 1317 (1994) by copyright permission of the Rockefeller University Press.");)
Human keratinocytes. Fluorescein concanavalin A and propidium iodide.
![Drosophila ovaria...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000458.jpg","Drosophila ovarian egg chamber.","Day 10 of development of a <i>Drosophila</i> ovarian egg chamber assembly line. The nuclei of follicle and nurse cells were labeled with propidium iodide (Cat. No. P1304MP, P3566, P21493) and visualized by confocal laser-scanning microscopy using excitation by the 568 nm spectral line of an ArâKr laser. Image contributed by Sandra Orsulic, University of North Carolina at Chapel Hill.");)
![Solemya reidi gil...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/g000661.jpg","Solemya reidi gill filaments.","Gill filaments from <i>Solemya reidi</i>—a gutless mollusk inhabiting the highly sulfidic sediments characteristic of pulpmill effluents and sewage outfall sites along the western coast of North America— is stained with either the SYTOX® Green nucleic acid stain (top; Cat. No. S7020, top), propidium iodide (middle; Cat. No. P1304MP, P3566, P21493), or DAPI (bottom; Cat. No. D1306, D3571, D21490. The ribbon-like bands of the gill lamella are composed of two cell types: granular cells, which harbor sulfur-oxidizing cells; endosymbiotic bacteria; and symbiont-free intercalary cells. The three images were acquired using a fluorescein bandpass optical filter set, a propidium iodide longpass optical filter set and a DAPI longpass optical filter set, respectively. The images were contributed by K. Warren and S. C. Cary, College of Marine Studies, University of Delaware.");)
![Jurkat human T-ce...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/A13201_TF_080828.jpg","Jurkat human T-cell leukemia cells treated with 1 µM camptothecin.","Jurkat human T-cell leukemia cells treated with 1 µM camptothecin. The externalized phosphatidylserine, a characteristic of early-stage apoptotic cells, was detected with Alexa Fluor® 488 annexin V (Cat. no. A13201). The late-stage apoptotic and necrotic cells were stained with propidium iodide (Cat. no. P1304MP, P3566, P21493). The image was acquired using bandpass filters appropriate for fluorescein.");)
Jurkat human T-cell leukemia cells treated with 1 µM camptothecin.
![Detection of prol...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/B23151_BrdU_080828.jpg","Detection of proliferation in Wil2S Lymphoma B cells.","Detection of proliferation in Wil2S Lymphoma B cells. Cells were treated with 10 µM 5-bromo-2´-deoxyuridine (BrdU, Cat. no. B23151) in culture medium for one hour, then pelleted and fixed with cold 70% ethanol. After treatment with RNase and 4 M HCl (to denature the DNA), the cells were labeled with anti-BrdU (Cat. no. A21300) and detected using green-fluorescent Alexa Fluor® 488 goat antiâmouse IgG antibody (Cat. no. A11001). In addition, the cells were labeled with red-fluorescent propidium iodide (Cat. no. P1304MP, P3566, P21493) to assess the total cellular DNA content. The cells were analyzed by flow cytometry using 488 nm excitation; the fluorescent signals were collected at ~525 nm for the Alexa Fluor® 488 dye and at ~675 nm for propidium iodide. Increased BrdU incorporation is indicative of actively proliferating cells.");)
![A cryostat sectio...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/P1304MP_TF2_080806.jpg","A cryostat section through adult zebrafish kidney that was incubated with the ELF® 97 phosphatase substrate in our ELF® 97 Endogenous Phosphatase Detection Kit and counterstained with propidium iodide.","A cryostat section through adult zebrafish kidney that was incubated with the ELF® 97 phosphatase substrate in our ELF® 97 Endogenous Phosphatase Detection Kit (Cat. no. E6601) and counterstained with propidium iodide (Cat. nos. P1304MP, P3566, P21493). The ELF® 97 alcohol signal, which is ordinarily green fluorescent, appears yellow in this section because the phosphatase activity is in close proximity to the nuclei, which are stained red by the propidium iodide. This double-exposure image was obtained using longpass filter sets appropriate for ELF® 97 alcohol product and tetramethylrhodamine.");)
A cryostat section through adult zebrafish kidney that was incubated with the...
![Lightly fixed adu...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/E6601_TF2_080806.jpg","Lightly fixed adult zebrafish intestine stained with the substrate contained in the ELF® 97 Endogenous Phosphatase Detection Kit.","Lightly fixed adult zebrafish intestine stained with the substrate contained in the ELF® 97 Endogenous Phosphatase Detection Kit (Cat. no. E6601). This kitâs novel ELF® substrate yields a green-fluorescent precipitate at the site of endogenous phosphatase activity. A partially digested brine shrimp, which also contains endogenous phosphatase activity, can be seen in the top left corner of this micrograph. The tissue was counterstained with propidium iodide (Cat. no. P1304MP, P3566, P21493).");)
Lightly fixed adult zebrafish intestine stained with the substrate contained ...
![Detection of BrdU...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/H1398_TF_080828.jpg","Detection of BrdU-incorporating cells using the SBIP technique.","Detection of BrdU-incorporating cells using the SBIP (Strand Breaks Induced by Photolysis) technique. Exponentially growing human promyelocytic leukemia (Cat. no. HL60) cells were incubated with 20 µM BrdU (Cat. no. B23151) for 40 minutes and then with 20 µg/mL Hoechst 33258 (Cat. no. H1398, H3569, H21491) in the presence of 2% DMSO for an additional 20 minutes. After this incubation, the cells were exposed to 300 nm UV light for 5 minutes to selectively photolyze DNA that contained the incorporated BrdU, and then fixed in 70% ethanol. Subsequent incubation of the permeabilized cells with the ChromaTide® BODIPY® FL-14-dUTP (Cat. no. C7614) in the presence of exogenous terminal deoxynucleotidyl transferase resulted in incorporation of the fluorophore into DNA strand breaks, thereby labeling the S-phase cells. The DNA was counterstained with the red-fluorescent nucleic acid stain propidium iodide (Cat. no. P1304MP, P3566, P21493); therefore, the BODIPY® FL dyeâlabeled DNA appears yellow. The image was contributed by Zbigniew Darzynkiewicz, Cancer Research Institute, New York Medical College.");)
Detection of BrdU-incorporating cells using the SBIP technique.
![Mouse Anti-Alpha ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/Mouse_Anti_Alpha_Tubulin_Monoclonal_Antibody07.jpg","Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)","Microtubules of fixed bovine pulmonary artery endothelial cells (BPAEC) were localized with mouse monoclonal antiâa-tubulin antibody (Cat. no. A11126), followed by the biotin-XXâconjugated F(ab\\\)
| Color: |
Red |
| Platform: |
Flow Cytometer, Fluorescence Microscope |
| Product Size: |
10 ml |
| Detection Method: |
Fluorescent |
| Cell Permeability: |
Cell-Impermeant |
| Sub-Cellular Localization: |
Cytoplasm & Cytosol |
| Shipping Condition: |
Room Temperature |
| Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures. |
Store in refrigerator (2–8°C) and protect from light.
Fluorescence Ex/Em spectra of propidium iodide bound to DNA.
Note: Use lot number only (e.g., "K18126").
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