Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions.
• Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
• Amplifies aRNA for gene array analysis and other applications
• Efficiently incorporates many modified nucleotides
A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase.
Accessory Products:
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript® technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, Ambion recommends using the MEGAclear™ Kit (SKU#AM1908) and the MEGAclear™-96 Kit (SKU#AM1909).
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![Transcription of ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00031.jpg","Transcription of Long Templates with the MEGAscript⢠Kit and a Competitor\\\)
![Effect of Templat...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00186.jpg","Effect of Template Size on Yield of MEGAscript⢠Reactions","20 µl transcription reactions containing 1 µg of different DNA templates coding for the indicated sized transcripts were added to each reaction. Inset shows 0.25 µl aliquots of both conventional and MEGAscript reactions transcribing a 0.3 kb transcript separated on a 2% agarose gel and ethidium bromide stained.");)
![Schematic of aRNA...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00289.jpg","Schematic of aRNA synthesis","");)
![MEGAscript⢠Yield...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00337.jpg","MEGAscript⢠Yield Comparison","A 4 kb template was used in transcription reactions with the MEGAscript Kit or two other commerciallyavailable kits as per protocol. Each 20 µl transcription reaction was diluted to 100 µl and 1 µl was assessed using the Agilent 2100 bioanalyzer. The area under the peak is indicative of yield.");)
![RNA Products From...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00401.jpg","RNA Products From Two Transcription Systems","1 kb, 4 kb or 9 kb templates were used in Ambion\\\)
![Mass Amounts of R...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00402.jpg","Mass Amounts of RNA Transcripts Produced by Two Distinct Transcription Systems","1 kb,4 kb or 9 kb templates were used in Ambion\\\)
![Electropherogram ...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f00403.jpg","Electropherogram of RNA Transcripts of Different Sizes From Two Transcription Systems","1 kb, 4 kb or 9 kb templates were used in Ambion\\\)
![MEGAscript® T7 Kit](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/f01402.jpg","MEGAscript® T7 Kit","");)
