The Qubit® (previously known as Quant-iT™) dsDNA BR Assay Kit is designed specifically for use with the Qubit® 2.0 Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.
Notes:
1. All Qubit® assay kits can also be used with the Qubit® 1.0 Fluorometer.
2. 500 µL thin-walled PCR tubes are required but not included.
![Qubit is specific...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/Selectivity%20of%20Qubit_2.JPG","Qubit is specific for DNA or RNA.","Selectivity of the Qubit® assays compared to UV spectroscopy. Triplicate samples containing lambda DNA (10 ng/µL) and varying amounts of ribosomal E. coli RNA (0â100 ng/µl) were assayed using Qubit® DNA BR and Qubit® RNA BR assays on the Qubit® 2.0 Fluorometer according to kit protocols. The same samples were subsequently measured in triplicate using a NanoDrop® ND-1000 Spectrophotometer, and single measurements were made using a Perkin Elmer Lambda 35 Spectrophotometer. The concentrations indicated are the concentrations of DNA and RNA in the starting samples, before dilution in the Qubit® assay tubes. The red and orange trendlines indicate the actual concentrations of DNA and RNA, respectively, in the starting samples. The actual concentration of nucleic acid was set by diluting pure, concentrated solutions of DNA and RNA to an optical density of 1.0 at 260 nm using a Perkin Elmer Lambda 35 Spectrophotometer. The concentrations of the stock solutions were then calculated and used for all subsequent dilutions. With UV analysis, results for samples containing both DNA and RNA are nondiscriminatoryâyou cannot distinguish one from the other.");)
