(Novex®)
| Reactivity: |
Mouse |
| Validated Application: |
Western Blot |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Target Gene: |
PKA Reg II beta [pS114] |
| Gene ID: |
5577 |
| Label or Dye: |
Unconjugated |
| Target Modification State: |
Phospho |
| Purification: |
Purified |
| Product Size: |
10 blots |
| Protein Family: |
Kinases & Inhibitors |
| Research Category: |
Virology |
| Shipping Condition: |
Approved for shipment on Wet or Dry Ice |
| Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures. |
Store in freezer (-5 to -30°C).
Note: Use lot number only (e.g., "K18126").
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![Up-regulation and...](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/44998-Final.jpg","Up-regulation and Antibody-Peptide Competition.","Peptide Competition and Phosphatase Treatment. Lysates prepared from 3T3-L1 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, and incubated with PKA regIIbeta [pS114] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate (Cat. no. ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that the peptide corresponding to PKA regIIbeta [pS114] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.");)
