| Promoter: |
MT |
| Vector Type: |
pMT, TOPO-TA Cloning Vectors |
| Product Size: |
20 reactions |
| Cloning Method: |
TOPO®-TA |
| Inducing Agent: |
Copper Sulfate |
| Protein Tag or Fusion: |
His Tag (6x), V5 Epitope Tag |
| Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures. |
Each DES® TOPO® TA Expression Kit contains two boxes. The DES® TOPO® TA box contains 200 ng of linearized and topoisomerase-activated pMT/V5-His- TOPO® vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a control
expression plasmid (pMT/lacZ). Store at -20°C. The One Shot® box contains high-efficiency, single-use 50 µl aliquots of competent TOP10 E. coli cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored.
| Vector Name | Map | Polylinker | Sequence | Restriction |
| pMT /LacZ |
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| pMT/V5-His-TOPO |
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Note: Use lot number only (e.g., "K18126").
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![Figure 1](javascript: toggle("http://tools.invitrogen.com/content/sfs/gallery/high/2030.jpg","Figure 1","");)
