With our patented att sites, the pCR®8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.
The pCR®8/GW/TOPO® TA Cloning Kit offers:
- att sites for rapid recombination into a variety of Gateway® destination vectors
- 3´-T overhangs for direct ligation of Taq-amplified PCR products
- Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
- Spectinomycin resistance gene for robust selection in E. coli
- EcoRI sites flanking the PCR product insertion site for easy excision of inserts
This kit includes One Shot® TOP10 Chemically Competent E. coli.