1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels.

  • Round, easy to remember band sizes
  • Broad size range (100bp – 12Kb)
  • Compatible with all agarose gels
Our Most Popular and Easiest to Use Ladder
1 Kb Plus DNA ladder is our most popular DNA ladder used to estimate molecular mass of double stranded DNA bands from PCR, restriction digests and other molecular biology protocols. Unlike some other DNA ladders, 1 Kb Plus DNA ladder has been specially designed to have bands of round, easy to remember sizes. The logical band lay out, and quick identification doublet, allow you to quickly and easily estimate the size of your band of interest at a glance. The migration pattern of 1 Kb Plus DNA ladder can be found in the image below (Fig 1).

A Single Ladder for Most Your Needs
The 20 bands comprising 1 Kb Plus DNA ladder range in size from 100bp up to 12 Kb making it one of the broadest DNA ladders available. This wide breadth is perfect for downstream cloning applications as well as end point PCR and other molecular biology applications.

Simplified Format for Use with All DNA Agarose Gels
1 Kb Plus DNA ladder is compatible with all agarose gels that can be used to analyze DNA fragments, including self cast agarose gels and E-Gel products. The ladder is provided in a purified format without pre-mixed loading buffer. The ladder should be mixed with an appropriate loading buffer prior to being loaded onto an agarose gel. 1 Kb Plus DNA Ladder is supplied at 1 µg/ µl in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 1 mM EDTA. Store at -20°C, and for best results aliquot stock to avoid repeated freeze-thaw cycles.

DNA Stain and Radiolabel Compatible
To visualize 1 Kb Plus DNA ladder stain with SYBR® Safe, ethidium bromide, or a similar DNA stain. 1 Kb Plus DNA ladder can also be radiolabeled using T4 polynucleotide kinase, T4 DNA polymerase, DNA polymerase I, or the large fragment of DNA polymerase I (Klenow fragment). Fill-in labeling can use any radiolabeled dNTP.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.