Applied Biosystems® TaqMan® Ribosomal RNA Control Reagents are designed to detect the 18S ribosomal RNA (rRNA) gene. The TaqMan® probe and primer sequences were designed following Rapid Assay Development Guidelines. The TaqMan® probe and primer sequences are conserved among a diverse group of eukaryotes including human, rat, mouse, Xenopus, Saccharomyces, Giardia, and Arabidopsis. Ribosomal RNA levels provide an endogenous control for PCR quantitation studies.
• Pre-designed primers and TaqMan® probe eliminate assay design
• Rapid Assay Development Guidelines can minimize optimization time
• Separate components provide flexibility in assay set-up
Comprehensive Guidelines Included
Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.
All Applied Biosystems® Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.
Notes on Use
The TaqMan® Ribosomal RNA Control Reagents can be used with TaqMan® Universal PCR Master Mix or TaqMan® PCR Core Reagents for polymerase chain reaction (PCR).
The TaqMan® Ribosomal RNA Control Reagents can be used with the TaqMan® One-Step RT-PCR Master Mix Reagents, TaqMan® EZ RT-PCR Core Reagents, or the TaqMan® Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).
Notice to Purchaser
Disclaimer of License: The TaqMan® Ribosomal RNA Control Reagents are optimized for use in the polymerase chain reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser by the purchase of these products. A license to use the PCR Process for certain research and development activities accompanies the purchase of certain Applied Biosystems® reagents when used in conjunction with an authorized thermal cycler, or is available from Applied Biosystems®. Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Director of Licensing at Applied Biosystems®, 850 Lincoln Centre Dr., Foster City, California 94404 or Roche Molecular System, Inc., 1145 Atlantic Avenue, Alameda, California 94501.
TaqMan® probes are covered by US Patent 5,723, 591 and foreign counterparts and patents pending owned by Applied Biosystems®, and may be covered by US Patents 5,801,155 and 6,084,102 and foreign counterparts licensed to Applied Biosystems®.
Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.