The Pippin Prep™ System is a small tabletop instrument that automates the size selection and collection of DNA for PGM™ sequencer libraries. Simply enter the desired base-pair target range via the software interface and after a 1.5 hr run the system will yield the programmed DNA fractions with virtually no low molecular weight contamination. The tight fragment size distributions obtained provide a more uniform template for amplification. And the processing of samples is simplified through the use of disposable pre-cast agarose gel cassettes.

Benefits of the Pippin Prep™ System

• Time savings compared to other methods
• Reproducible extractions offer more consistent results
• Narrow fragment size distributions permit more uniform colonies
• Minimal low molecular weight contamination reduce wasted colonies
• Individual sample channels minimize sample cross-contamination

Components of the Pippin Prep™ System:

The Pippin Prep™ electrophoresis instrument, which includes optics for the detection of fluorescently labeled DNA and an on-board computer with software interface.

Pippin Prep™ Kits, which contain 10 disposable pre-cast agarose gel cassettes and a reagent pack. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in the following formats:

SKUProduct NameCompositionEffective Range
4472170Pippin Prep™ Kit 20102% agarose100-600 bp
4472171Pippin Prep™ 2010 CEF2% agarose
Ethidium-free		100-600 bp
4472172Pippin Prep™ Kit 30103% agarose50-300 bp


A Streamlined Process
The workflow for Pippin Prep™ DNA size selection consists of just a few simple steps: prepare the cassette, load 4 samples and 1 size standard into the 5 wells of the cassette using standard pipetters and tips, enter a base-pair target range via the software interface, and press "start". During the 1.5 hr run the DNA will be separated by size and collected into 45 µl fractions. Simply return at the end of the run and collect the fractions.

Note: See user's manual or package insert for limited label license, and trademark information. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. Pippin Prep is a trademark of Sage Science, Inc.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.