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pcDNA™3.1 Directional TOPO® Expression Kit

pcDNA™3.1 Directional TOPO® Expression Kit

(Invitrogen™)

Description

The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression.

Directional Cloning technology facilitates expression experiments because:

A proofreading enzyme is used for fewer errors in cloned genes
Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation

In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.

Catalog Number

K4900-40

Size: 40 reactions

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Your Price: (USD) 1,207.00

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Specifications

General Specifications

Promoter:	CMV
Protein Tag or Fusion:	His Tag (6x), V5 Epitope Tag
Constitutive or Inducible System:	Constitutive
Delivery Method:	Transfection
Cloning Method:	Directional TOPO®
Key Function:	Constitutive Expression
Selection Agent (Eukaryotic):	Geneticin® (G-418)
Vector Type:	Directional TOPO Vectors, pcDNA
Product Size:	40 reactions
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Contents and Storage

Each pcDNA™3.1 Directional TOPO® Expression Kit contains two boxes. The TOPO® box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid. Store at -20°C. The One Shot® box contains single-use 50-µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. Guaranteed stable for 6 months when properly stored.